Molecular mechanism of force generation by dynein, a molecular motor belonging to the AAA+ family.
نویسندگان
چکیده
Dynein is an AAA+ (ATPase associated with various cellular activities)-type motor complex that utilizes ATP hydrolysis to actively drive microtubule sliding. The dynein heavy chain (molecular mass >500 kDa) contains six tandemly linked AAA+ modules and exhibits full motor activities. Detailed molecular dissection of this motor with unique architecture was hampered by the lack of an expression system for the recombinant heavy chain, as a result of its large size. However, the recent success of recombinant protein expression with full motor activities has provided a method for advances in structure-function studies in order to elucidate the molecular mechanism of force generation.
منابع مشابه
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 36 Pt 1 شماره
صفحات -
تاریخ انتشار 2008